Catalog Number - 75001-SP250

75001-SP250 Cogent HPS Silica™ Semi‑Prep HPLC Column is packed with high‑purity, unmodified silica, delivering highly consistent and reproducible performance for normal phase purification of polar and hydrophilic compounds.

Because the stationary phase is not chemically modified, it enables strong direct interactions with polar analytes, making it ideal for semi‑preparative purification of fats, lipids, and other hydrophilic compounds.

This proprietary HPS Silica™ phase has been widely used in HPLC laboratories for many years as a premium-value alternative to well-known silica columns such as Inertsil® and Luna®, providing dependable selectivity and long-term reliability.

The 5 µm particle size is optimized for semi‑prep work, providing:

• Lower backpressure compared to smaller particles

• Higher allowable flow rates

• Durable performance under repeated purification cycles

250 mm Length – Maximum Resolution for Semi‑Prep Purification

The 250 mm length provides the highest resolving power in this semi‑prep format.

• Maximum resolution compared to 50 mm, 100 mm, and 150 mm columns

• Superior separation of closely related or co‑eluting compounds

• Increased retention and peak capacity for complex mixtures

✅ Recommended for final purification, impurity isolation, and highly complex separations

10 mm ID – Semi‑Preparative Scale

• Designed for semi‑preparative purification workflows

• Increased sample loading capacity

• Suitable for analytical‑to‑prep scale‑up

• Compatible with fraction collection systems

✅ Recommended for process development and higher‑purity isolation workflows

Flow Rate & System Requirements (10 mm ID, 5 µm)

⚠️ Requires semi‑preparative HPLC system capability

• Typical flow rate range: ~4 – 12 mL/min

• Moderate backpressure (increased with 250 mm length)

• Designed for high‑flow purification

• Compatible with preparative detectors and fraction collectors

✅ Important: A specialized HPLC system is required for columns of this size

Why Choose HPS Silica™ for Semi‑Prep

High‑Purity Unmodified Silica

• Strong retention of polar compounds

• Ideal for normal phase purification

USP L3 Compatibility

• Suitable for compendial methods

• Reliable alternative to legacy silica phases

Proven Performance

• Widely used across HPLC laboratories

• Consistent and reproducible results

Performance Advantages

Low Backpressure (5 µm Particles)

• Enables higher flow rates

• Compatible with preparative systems

Fast Equilibration

• Improves throughput and reduces downtime

Strong Polar Retention

• Ideal for lipids and hydrophilic analytes

Typical Applications

• Semi‑preparative purification of fats and lipids

• High‑resolution normal phase separations

• Impurity isolation

• Polar compound purification

• Pharmaceutical and chemical production workflows

Important Usage Notes

• Designed for normal phase chromatography conditions

• Use appropriate non‑polar mobile phases

• Requires semi‑preparative HPLC system capability

• Increased pressure expected due to longer column length

• Ensure proper equilibration for reproducibility

Made‑to‑Order Columns & Pricing Information

This is a custom, made‑to‑order column.

• Columns are manufactured specifically to order

• Orders cannot be canceled once placed

• Custom columns are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification: Class A Custom Item

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.